Mapping the distribution and trends of co-circulating dengue virus serotypes in Odisha, India: A retrospective facility-based analysis

INTRODUCTION

Dengue, a member of the Flaviviridae family, is one of the major emerging public health concerns in India but we do not have a specific antiviral drug against it. Though, the virus prevails for a long time, only prevention and control of the virus is targeted, and eradication seems a daunting target. In a short span, the dengue infection has rapidly spread from tropical to subtropical areas with around 2.5 billion people living in the dengue-risk zone.¹

The dengue infection among humans is caused by Aedes aegypti mosquito, which preferentially rests in dark and cool closets, and generally tends to bite humans indoors. They lay eggs on the sides of water-holding containers such as tyres. These eggs hatch into larvae following rains or floods. Favourable environmental conditions help in transforming larvae into pupae followed by adult mosquitoes, in little over a week. It takes a mean of 15 days (5–33 days) for the virus to multiply, mature, and migrate to the salivary glands before the mosquito can transmit it to humans.^2,3

The dengue virus (DENV) has four predominant serotypes: DENV1, DENV2, DENV3 and DENV4;⁴ though a fifth serotype that is closely related to DENV4 has also been reported in 2013.⁵ Patients with dengue infection manifest a high-grade fever (40 °C/104 °F) accompanied with other flu-like symptoms such as headache, pain behind eyes, nausea, vomiting, myalgia, arthralgia and rash. The incubation period of dengue is 4–10 days. The disease is a continuum that ranges from mild to severe forms, which can also be fatal.^2,6 Repeated exposures to dengue virus may also lead to dengue haemorrhagic fever (DHF), which manifests more aggressively compared to dengue fever. The clinical presentation of DHF includes severe abdominal pain, bruising or bleeding under the skin, clammy or cold skin, nose bleeding, a sudden decrease in the blood pressure, haematemesis, black stool and capillary leakage.⁷ There is a short-lived, cross-protective immunity to heterologous serotypes for months post-exposure.⁸ Dengue virus-specific immunoglobulin (IgM) antibodies appear as early as around 3 days of dengue viral fever and can persist for 30–60 days, whereas IgG antibodies appear at about day 7, peak at 2–3 weeks and persist for life.⁹

Not much is known about the geographical distribution of type-specific dengue virus in Odisha as dengue serotyping is not done routinely. Additionally, each dengue serotype exhibits varied levels of severity and different symptoms. There is a need to understand the patterns of dengue spread through serotyping to facilitate knowledge of endemicity and assist public health efforts for prevention and control of dengue. We aimed to map the distribution and co-circulation of currently prevalent dengue serotypes in Odisha.

METHODS

Study design and setting

A retrospective cross-sectional study based at the Regional Medical Research Centre, Bhubaneswar was done through the dengue samples received in 2018. The Regional Virology Research and Diagnostic Laboratory (VRDL) of this institute is the diagnostic centre for various viral diseases including dengue where samples of suspected dengue cases are referred for testing from entire Odisha.

Odisha is located between 17.78°N and 22.73°N latitudes, and 81.37°E and 87.53°E longitudes. The total area of the state is 155 707 km² which includes coastal plains in the east and hills in the west. Four meteorological seasons: winter (January– February), pre-monsoon (March–May), southwest monsoon (June–September) and northeast monsoon (October–December) are experienced in the state. This climatic pattern favours mosquitoes to survive easily making Odisha a dengue-prone area.¹⁰

Topographically, Odisha is divided into four regions.¹¹ The first is the northern plateau, a predominantly hilly area with an elevation ranging from 2000 to 3000 feet above sea level; the second is a coastal belt consisting of a number of river deltas; the third is the central table land with flat, slightly surge and folded topography with an elevation around 1000 feet; and the fourth is the eastern ghats with elevated plateaus ranging from 900 to 2000 feet dominated by hills with valleys and plains lying between them. Our study included samples from 18 of 30 districts of Odisha covering all the four topographical regions (Fig. 1).

Close

FIG 1.

Topographical representation of Odisha

Export to PPT

RESULTS

A total of 2892 serum samples were received from 18 district hospitals across Odisha during January–December 2018. These samples were subjected to confirmation of dengue virus and identifying serotype. After the diagnosis, only NS1 antigen-positive samples were considered for serotyping and analysis. Of the 2892 samples tested, 763 (26.4%) were positive for NS1 antigen by ELISA. The samples received from Angul, Balangir, Baragarh, Dhenkanal (Central Table Land); Mayurbhanj (Northern Plateau), Khorda, Nayagarh, Puri, Bhadrak, Cuttack, Balasore, Jagatsinghpur, Kendrapada, Ganjam, Jajpur (Coastal belt) and Rayagada, Kalahandi, Kandhamal (Eastern Ghats) were positive for dengue virus.

Among 2892 samples received, 1742 (60.2%) patients were men and 1150 (39.8%) were women. A total of 763 individuals were found to have antibodies against NS1 dengue virus antigen and 260 patients had IgM antibodies to dengue virus. The gender distribution of patients having antibodies to NS1 antigen is shown in Fig. 2. There was no significant difference observed in NS1-positivity rate between genders.

Close

FIG 2.

Gender distribution of dengue affected individuals and their NS1 antigen positivity

Export to PPT

We observed a seasonal variation as most cases of dengue virus occurred during June to November, with a peak around August. The positivity rate of dengue varied from 20% to 34% across the seasons. Additionally, the dengue-positivity rate had consistent patterns with seasonal variation (Fig. 3).

Close

FIG 3.

Month-wise distribution of dengue affected individuals and their NS1 antigen positivity

Export to PPT

Antibodies to NS1 antigen among the infected individuals were found to be higher among those aged 11 to 50 years. The peak antigen positivity 30.5% (Fig. 4) was observed among those aged 31–40 years.

Close

FIG 4.

Age-wise distribution of dengue affected individuals and their NS1 antigen positivity

Export to PPT

All four serotypes of dengue, namely DENV1, DENV2, DENV3, and DENV4, were detected in the samples. The observed base pair sizes were DENV1-482 bp, DENV2-119bp, DENV3-290bp and DENV4-392bp, respectively. Only few districts such as Rayagada, Balangir and Kalahandi had mixed serotypes (Table I). Serotyping could not be done for 5 of 18 districts as very few samples were received from these districts. Further, among these samples, dengue-positive cases were less and few could not be processed due to low sample quantity/volume.

DISCUSSION

Dengue is an emerging disease of international concern.¹³ During the past few decades it has spread across vast geographical regions including urban areas.¹⁴ The key factors responsible for rapid spread of dengue are urbanization and frequent travel including international travel. The first dengue outbreak in Odisha was reported during 2010 from Bhubaneswar.¹⁰ Following this outbreak, cases continued to be reported from the entire state. We screened for dengue and serotyping of the virus was done to map the prevalent dengue serotypes across the state. Previous studies on dengue serotypes in Odisha reported serotypes either in a particular district or as a part of other investigations. To the best of our knowledge, this is the first study from this region that summarizes dengue serotypes across all topographies and seasons making the evidence comprehensive.

Previous reports suggest DENV1, DENV2 and DENV3 serotypes of dengue to be prevalent in Odisha.^15,16 We observed DENV2 and DENV3 to be the most dominant circulating serotypes, with a few districts having mixed serotypes as well. These mixed serotypes DENV1 and DENV2 in Kalahandi; DENV2 and DENV3 in Rayagada; and DENV1 and DENV3 in Balangir were heterogeneously distributed, which might pose a challenge for disease control in the respective areas.

Annual seasonal variation of dengue revealed that the infection appears abruptly around July, often at the start of the rainy season with a peak in August and September, the most favourable months for mosquito breeding, which is consistent with the findings of other studies.¹⁷ This seasonal trend needs to be considered for preparedness and public awareness programmes to control future outbreaks. Our study showed that men (61%) are more likely to have dengue infection than women. Also, young people aged 21–40 years were more prone to dengue infection in comparison to other age groups, which is consistent with the findings of other studies.^18,19 One of the reasons for this pattern could be that men and young people often work outdoors, thus exposing them to mosquito bites.

In 2018, DENV2 dengue serotype was predominant in Odisha. Further studies are required for better understanding of the epidemiology of disease. We suggest continuous surveillance to monitor trends in the characteristics of circulating dengue serotypes for better understanding of the endemicity and severity of the disease. This will help in strengthening public health strategies to control the disease including deployment of resources.